Kanazawa University Research

Lipids and Proteins: Further Evidence for Functional Crosstalk in Cells - Seite 2

2-Cys Prxs was subsequently isolated with utmost care, ensuring it was free of all lipids. In a serendipitous discovery, the team now noticed that Mg-ADP or overoxidation no longer induced HMWs. Could it be the lipids then, that led to formation of HMWs? Four different membrane lipids were now incubated with 2-Cys Prxs. Two of these, phosphotidylglycerol (PG) and phosphotidylserine (PS) did indeed result in HMWs formation. This too had a nearly identical spherical structure, containing trefoil-shaped particles, most likely oligomers of 2-Cys Prxs. To assess functionality of the newly formed HMWs, their chaperone activity was tested on an artificially distressed protein. Indeed, only chaperone activity and no inherent peroxidase activity was seen.

The authors speculate the exact chaperone function of the HMWs in the context of stress. They hypothesize that this complex might play a role in capturing and removing incorrectly structured proteins from the cell, which would otherwise cause more damage. Although this hypothesis needs further validation, their discovery of the specific lipids involved in this mechanism, will make it much easier. Given the need for chaperones in combatting aging, neurodegenerative diseases and cancer, these findings could have an impact on therapeutic research.

References:

Takamitsu Haruyama, Takayuki Uchihashi, Yutaro Yamada, Noriyuki Kodera, Toshio Ando and Hiroki Konno, "Negatively Charged Lipids Are Essential for Functional and Structural Switch of Human 2-Cys Peroxiredoxin II", Journal of Molecular Biology (January 2018)

doi: https://doi.org/10.1016/j.jmb.2017.12.020

About WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University

The Kanazawa University Nano Life Science Institute (WPI-NanoLSI) was launched October 2017 following the selection of The University for the World Premier International Research Initiative (WPI) by Japan's Ministry of Education, Culture, Sports, Science and Technology (MEXT).

The mission of the NanoLSI is to combine the world's foremost knowledge of bio-scanning probe microscopy and supramolecular chemistry to develop 'nano-endoscopic techniques' to directly image, analyze, and manipulate the nano-dynamics of proteins and nucleic acids both on the surface and inside of living cells. Notably, complementary experimental and multi-scale simulation techniques developed in this research will form a basis for elucidating wide ranging molecular and cellular dynamics by comparing healthy and cancer cells.

Based on the techniques and expertise gained through this process, Kanazawa University will create the new academic field of 'Nanoprobe Life Science', to promote fundamental understanding of critical mechanisms governing diverse life phenomena such as diseases and aging.

https://nanolsi.kanazawa-u.ac.jp/en/

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