Bruker Launches timsTOF HT with Greater Dynamic Range for Further Enhancing Unbiased, High-Confidence 4D Proteomics

At the 70^th ASMS meeting, Bruker Corporation (Nasdaq: BRKR) today announced the further evolution of the revolutionary 4D Multiomics timsTOF platform with the launch of the new timsTOF HT system. The timsTOF HT includes a novel 4^th-generation TIMS (trapped ion mobility separation) XR cell and 14bit digitizer for even greater dynamic range, enhanced peptide coverage and more accurate quantitation, particularly in unbiased 4D plasma and tissue proteomics and epiproteomics.

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timsTOF HT instrument (Photo: Business Wire)

These advances are achieved without compromising the ultra-high sensitivity and extreme robustness for high-throughput proteomics, e.g., at 50 samples per day (SPD) or up to 200 SPD, or the high scientific confidence of better than 1% peptide and protein FDRs (false discovery rates), avoiding inherent antigen cross-reactivity in targeted immune-recognition methods. The timsTOF HT instrument can identify over 100k unique tryptic peptides in 60min gradients with accurate quantification of better than 5% CVs for many protein groups in low microgram quantities of a three-proteome mixture, using the dia-PASEF technique. The timsTOF HT also is optimized for high-throughput, deep and unbiased plasma proteomics and liquid biopsy biomarker research.

Professor Florian Meier from the University of Jena said: “Our ongoing collaboration with Bruker to stream-line tissue analysis is a key area in clinical proteomics, yet extremely challenging as tissue sections and biopsies comprise very heterogenous cell populations. The dia-PASEF acquisition mode on the timsTOF HT instrument quantifies proteins across a large dynamic range even in notoriously difficult samples, such as cardiac tissue, without sacrificing throughput or sensitivity.”

Dr. Bruce Wilcox, Vice President of Proteomics at PrognomiQ Inc., uses the Seer Proteograph and the timsTOF Pro 2 for deep, unbiased plasma proteomics in the discovery of biomarkers in a pancreatic cancer study. Dr. Wilcox stated: “We detected 3822 protein groups across all 5 Seer nanoparticles for the 193 pancreatic cancer and healthy subject samples. Some 2933 protein groups were identified in at least 25% of the patient samples, using our several timsTOF systems.”