Bruker Advances CCS-Enabled 4D-Proteomics timsTOF Solutions for Immunopeptidomes and Glycoproteomics at US HUPO - Seite 2

B. Peak timsTOF Performance using TwinScape

TwinScape is a new digital-twin performance monitoring and quality control engine, leveraging iRT peptide standards to support sustained peak performance in sample prep and LC-MS. For synergistic multi-omics studies in the post-genomic era, a combination of depth of coverage, robustness, and scalable quantitation is required. TwinScape enables larger controlled studies and valid cross-lab comparability.

Dr. Anne-Claude Gingras, VP of Research at Sinai Health, Director at Lunenfeld-Tanenbaum Research Institute, and Professor in Molecular Genetics in Toronto, Canada said: “We support a variety of projects in our proteomics facility. It is critical that our instruments are at peak performance and that we always have a pulse on them. The combination of Bruker ProteoScape, TwinScape and the Biognosys iRT kit have become integral for monitoring our instruments to ensure everything is operating up to highest standards. TwinScape is great for an over-time view of QC and system performance. It has been especially useful in pinpointing if there ever is a sample, LC or MS problem.”

C. New glyco-PASEF for Ultra-High Sensitivity 4D-Glycopeptide Analysis

Bruker presents the novel timsTOF glyco-PASEF method with polygon filtering developed with the Heck Group at Utrecht University, the GlycoScape software developed collaboratively with the Van Gool Group at Radboud University, and MSFragger-Glyco software developed by the Nesvizhskii Group at the University of Michigan.

Dr. Albert Heck, Professor of Chemistry and Pharmaceutical Sciences at Utrecht University and Scientific Director of the Netherlands Proteomics Center, noted: “I am pleased with our pioneering work in leveraging the unique strengths of the timsTOF platform for glycoproteomics, now translated to the glyco-PASEF method for research in glycobiology.”

Bruker is now offering GlycoScape real-time glycoproteomic software, developed with Alain van Gool’s team at Radboud University, for early access beta-testing.

Bruker also announces that the MSFragger-Glyco software now supports timsTOF data. Dr. Alexey Nesvizhskii, Professor of Computational Medicine, Bioinformatics and Pathology at University of Michigan, and creator of MSFragger, stated: “My team is gratified by the popularity of MSFragger for ultrafast searches, including the identification of post-translationally modified peptides. Our software suite supports the analysis of PASEF data, and we are delighted that Bruker now offers it with timsTOF instruments. This recognition acknowledges the broad applicability of our tools to timsTOF-generated proteomics data.”